Aim: The aim of this study was to determine differences between generations of nurses regarding their personal and professional values. Material and Method: A descriptive and comparative study was conducted in a university hospital in Izmir, Turkey. The study was conducted with 164 nurses who accepted to take part in the study. Eighty five nurses that were born between 1965-1979 coded as X generation and 79 of them were coded as Y generation as they were born between 1980-1999. Data were collected by conducting a face to face interview by using Individual Characteristics Form, Portrait Values Questionnaire and Nurses' Professional Values ScaleRevised. Descriptive statistics, chi-square, Mann-Whitney U test and spearman correlation analysis were used in the analyzation of the data. Results: 51.8% of nurses were in X generation and 48.2% of them were in Y generation (n=164). In X generation, 96.4% of them were women, 52.9% of them were graduated from university and 72.6% of them were married. In Y generation, 93.7% of them were women, 51.9% of them were graduated from university and 58.2% of them were married. There was no statistically significant difference between these X and Y generation in terms of these characteristics. The difference between the mean scores of nurses at the X and Y generation on Nurses' Professional Values Scale and Portrait Values Questionnaire was not statistically significant. It was determined that there was a significant positive correlation between the mean scores of the Nurses’ Professional Values Scale and the Portrait Values Questionnaire of X and Y generation nurses who participated in the study. Conclusion: In the study, it was determined that there was no difference between X and Y generation nurses regarding their personal and professional values. It was thought that working together, continuous communication and interaction between X and Y generations resulted in these results.