Aim: This study was carried out semi-expermentally type to assess the effect of training given before the Pilonidal Sinus surgery on the anxiety and comfort levels of patients.Material and Methods: A total of 70 patients selected through sealed envelope method composed the cross-section of the research. Personal identification form, State-Trait< Anxiety Inventory, General Comfort Scale, patient-training leaflet and behavior control scale used for collect data. Percentage, average, t-test, Mann Whitney U test and ANOVA test used in the data analysis for the study. Significance level was accepted as p< 0.05. Results: A significant distinction didn’t found out in the study between the State-Trait Anxiety Inventory score averages of experimental and control group of patients (p>0.05). A significant distinction determinated between the average scores in the relaxation and dealing with problems, two of the sub-dimension of comfort questionnaire (p>0.05), but a significant difference has been found between the relief score averages, which is also a sub-dimension of comfort questionnaire (p<0.05). However, the patients in the experimental group were observed to show better attitudes such as consuming more fluid and keeping the operation zone dry when compared to control group (p<0.05). Conclusion: It deduced that the training given before the pilonidal sinus operation does not have an effect on the anxiety and comfort states. In this manner, it is suggested that education be done right time in before and after surgery and evaluation of comforts.