Objective : This descriptive study conducted in a state hospital to understand female health care professionals’ knowledge and practice level of Breast Self-Examination (BSE). Materials and Methods: Study sample was 76 voluntary women working in the hospital from July 9th to September 17th in 2009. Data of the study has been collected using a questionnaire developed by the researcher according to the literature. The participation was totally on the basis of voluntariness and the questionnaire has been filled up by the participants themselves. Evaluation of the data was done with computer. Results: Study found the 86.8% of them know how to perform BSE; 19.7% were doing it periodically each month. Signs and symptoms to observe through it were known between 32.9% - 60.5% and the awareness about the important points of it was between 30.3% - 77.6%. Conclusion : Research data analysis showed they know theoretically how to practice it but few performing it periodically and effectively and they have medium knowledge about what signs and symptoms to observe through breast self-examination.