Objective: This study is conducted to determine the factors affecting the anxiety and stress levels of first year midwifery students begining and after their first clinical experience. Method: This descriptive and analytical study used a popultaion of 75 students studying in the first grade of the midwifery department of the Health Sciences Faculty of a public university. Sample selection was not made in the study, and 70 students who agreed to participate in the study constituted the sample of the study (participation rate: 93%). The data were collected by "Personal Information Form", "Clinical Stress Questionnaire", "State-Trait Anxiety Inventory". Results: The average age of students participating in the study was determined as 18.92 ± 1.04. The levels of stress begining the first clinical applications of midwifery students were higher than later. Among the stress factors reported by the students were the fear of harm to the patient and the attitudes of the clinical physician-nurses. The state of the state-trait anxiety scores of the clinical application order was found to be significantly higher than that of the post clinical application. The state anxiety levels of students who voluntarily selected midwifery department and the stress level and state anxiety levels of students graduated from health vocational high school were found to be significantly lower. Conclusions: It has been found that, the state-trait anxiety and stress levels of the students rise begining their first clinical experience. Additionally, as the stundets’ clinical experience increase, their stress levels decrease.