Aim: This study has been performed to evaluate the effect of the applied-education in the ambulance simulation laboratory on the skill level of students registered in the Emergency and First Aid Program of Health Services Vocational School. Material and Methods: 67 students, registered in junior class of the Emergency and First Aid Program of Health Services Vocational School in 2013-2014 academic year, were admitted to the study conducted with half experimental model. Student questionnaires and “Skills Assessment Form" prepared by researchers which contained descriptive features of students were used as the data collection tool. Basic skill levels of students, related to the intervention for the patient in the ambulance, were evaluated pre and post-simulation education using the “Skills Assessment Form”. Simulation applications were performed under the supervision of the instructor with repeated intervals. Results: Female percentage were 61.2%, male percentage were 38.8% and the mean age were about 18.67 ± 1.18 for the students participated in the study. 85.1% of the students were the health vocational high school graduates, percentage of the working students and employees in an institution were 40.3%. 40.7% of working students were employed in 112 and 14.8% were employed as emergency medical technician in a hospital. When pre and post-education performances of students in "opening the pale road" and "Heart massage application" were compared it was found that the difference was statistically significant (p<0.001) and they performed the operation steps more accurately after the education. Conclusion: Accuracy of performing the basic life support applications of students were improved in all steps following the simulation education. As a result, it can be concluded that skill levels of students have been increased due to the education with simulation.